Coding

Part:BBa_K4585001:Design

Designed by: Siyao Ma   Group: iGEM23_CSU-CHINA   (2023-09-22)


GAL4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 134


Design Notes

GAL4 combining domain is universal among eukaryotic cells, but the activation factor needs to be chosen differently according to the chassis creature.


Source

GAL4 is a common part in double yeast hybrid system. It can recognize and combine with UAS. Because GAL4 is originated from yeast cells, the activation domain can not work in mammalian cells. Therefore, we only used its combining domain and use it together with VP64 which is an activation domain to realize the activation of downstream UAS transcription.

References